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IDT?成立于 1989年，是基因組學領域開發的領先者，也是公認的定制核酸生產行業的領導者。IDT 憑借在 DNA 合成領域的領導能力，為基因組學應用開發了專有技術，例如下一代測序、CRISPR 基因組編輯、合成生物學、數字 PCR 和 RNA 干擾。通過 GMP 服務，IDT的產品被科學家用于研究多種癌癥以及大多數遺傳性和傳染性疾病。IDT?在亞洲、歐洲和北美洲設有工廠，為一百多個國家及地區的超過十二萬名生命科學界研究人員提供服務，每天生產的寡核苷酸數量?超過七萬份。

北京澤平科技有限責任公司成立于2003年3月，一直致力為中國用戶提供全球最先進的生物試劑、科學儀器、實驗室消耗品、化工原料及醫藥中間體等技術服務。品牌成立至今，已與近百家國際一線技術供應商品牌建立了長效穩定合作機制，產品技術服務囊括近20w多個品類。立足北京，放眼全球，澤平科技“以最先進技術服務用戶”為己任，國內服務用戶已超過20000家。澤平科技和IDT公司達成合作，將進一步為用戶開放CRISPR基因組編輯等的產品服務。歡迎廣大用戶咨詢。

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技術優勢

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技術產品方案：

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以上產品請微信搜索“澤平科技”公眾號進入咨詢。

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參考文獻：

• Kim KW, Tang NH, et al.?(2018)?A neuronal piRNA pathway inhibits axon regeneration in?C. elegans.?Neuron,?97?:?1–9.
• Tr?der SE, Eber LK, et al.?(2018)?An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes.PLoS One,?13?(5)?:?e0196891.
• Brinkman EK, Kousholt AN, et al.?(2018)?Easy quantification of template-directed CRISPR/Cas9 editing.?Nucleic Acids Res.?doi: 10.1093/nar/gky164
• Gregg E. Homanics.?(2018)?Gene edited CRISPy critters for alcohol research.?Alcohol.?doi: 10.1016/j.alcohol.2018.03.001
• Andersson M, Turesson H, et al.?(2018)?Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery.?Physiol Plant.?doi: 10.1111/ppl.12731
• Ohtsuka M, Sato M, et al.?(2018)?i-GONAD: a robust method for?in situ?germline genome engineering using CRISPR nucleases.?Genome Biol,?19?:?25.
• Riddle MR, Aspiras AC, et al.?(2018)?Insulin resistance in cavefish as an adaptation to a nutrient-limited environment.?Nature,?555?:?647–651.
• Seki A, Rutz S.?(2018)?Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.?J Exp Med.?doi: 10.1084/jem.20171626
• Han X, Liu Z, et al.?(2017)?Cas9 ribonucleoprotein delivery via microfluidic cell-deformation chip for human T-Cell genome editing and immunotherapy?.?Adv Biosys,?1?:?1600007.
• Al Abdallah Q, Ge W, Fortwendel JR.?(2017)?A simple and universal system for gene manipulation in?Aspergillus fumigatus:?in vitro-assembled Cas9 guide RNA ribonucleoproteins coupled with microhomology repair templates.?mSphere,?2?:?e00446–17.
• di Pietro F, Valon L, et al..?(2017)?An RNAi screen in a novel model of oriented divisions identifies the actin-capping protein Z β as an essential regulator of spindle orientation.?Curr Biol,?27?:?2452–2464.
• Nachmanson D, Lian S, et al.?(2017)?CRISPR-DS: An efficient, low DNA input method for ultra-accurate sequencing.?bioRxiv.?doi: 10.1101/207027
• Schwinn MK, Machleidt T, et al.?(2017)?CRISPR-mediated tagging of endogenous proteins with a luminescent peptide.?ACS Chem Biol.doi: 10.1021/acschembio.7b00549
• Xu MM, Pu Y, et al..?(2017)?Dendritic cells but not macrophages sense tumor mitochondrial DNA for cross-priming through signal regulatory protein α signaling.?Immunity,?47?:?363–37.
• Mikheikin A, Olsen A, et al.?(2017)?DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle.?Nat Commun,?8?:?1665.
• Quadros RM, Miura H, et al.?(2017)?Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins?.?Genome Biology,?18?:?92.
• Wefers B, Bashir S, et al.?(2017)?Gene editing in mouse zygotes using the CRISPR/Cas9 system.?Methods,?121–122?:?55–67.
• Rivera-Torres N, Banas K, et al..?(2017)?Insertional mutagenesis by CRISPR/Cas9 ribonucleoprotein gene editing in cells targeted for point mutation repair directed by short single-stranded DNA oligonucleotides?.?PLoS One,?12?:?e0169350.
• Agudelo D, Duringer A, et al..?(2017)?Marker-free coselection for CRISPR-driven genome editing in human cells.?Nature Methods,?14?:615–620.
• Luo L, Bokil N, et al..?(2017)?SCIMP is a transmembrane non-TIR TLR adaptor that promotes proinflammatory cytokine production from macrophages?.?Nat Commun,?8?:?14133.
• Cuellar TL, Herzner AM, et al.?(2017)?Silencing of retrotransposons by SETDB1 inhibits the interferon response in acute myeloid leukemia.?J Cell Biol,?216?:?3535–3549.
• Jacobi AM, Rettig GR, Turk R, Collingwood MA, Zeiner SA, Quadros RM, Harms DW, Bonthuis PJ, Gregg C, Ohtsuka M, Gurumurthy CB,?Behlke MA.?(2017)?Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes.?Methods,?121–122?:?16–28.
• Kohler S, Wojcik M, et al..?(2017)?Superresolution microscopy reveals the three-dimensional organization of meiotic chromosome axes in intact?Caenorhabditis elegans?tissue?.?Proc Natl Acad Sci USA,?114?:?E4734–E4743.
• Grahl N, Demers EG, et al..?(2017)?Use of RNA-protein complexes for genome editing in non-albicans Candida?species?.?mSphere,?2?:e00218-17.

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